WebpTrcHis-TOPO (linearized) Linearized bacterial vector with 3'-T overhangs and bound topoisomerase, for TOPO® TA cloning and inducible expression of N-terminally 6xHis … WebDec 8, 2000 · Full-length PCR products were cloned into the pTrcHisTOPO vector (Invitrogen) exactly according to the manufacturer's protocols. Subclones were analyzed by automated DNA sequencing to verify sequence and ensure the fidelity of the reading frame for expression from the pTrcHisTOPO vector. Both strands of each cDNA clone were …
pTrcHis2 TOPO TA Expression Kit from Thermo Fisher Scientific
WebJul 7, 2003 · The plasmids pGEX-gp78c and pGEX-MmUBC7 were provided by A. Weissman (National Cancer Institute, Bethesda, MD). Genes coding for yeast Ubc13 and Mms2 were amplified by PCR from yeast genomic DNA and cloned into the pTrcHis-TOPO ® vector (Invitrogen). Wild-type Cdc48 was cloned into pYC2NT (Invitrogen), and amino acids E315 … WebThe pTrcHis- and pTrcHis2-TOPO TA Expression Kits allow fast, efficient cloning of PCR products into a prokaryotic expression vector for high-level, regulated expression from the trc promoter. The vector in each kit, pTrcHis-TOPO or modern physics by beiser
Producing HGH hormone via E. coli - MuscleChemistry
WebDie pTrcHis- und pTrcHis-TOPO™ TA-Expressionskits enthalten jeweils zwei Boxen. Die TOPO™ TA-Box enthält 200 ng Topoisomerase I-aktivierten pTrcHis-TOPO™ oder pTrcHis2-TOPO™ Vektor, steriles Wasser, dNTPs, 10x PCR-Puffer, ein Kontroll-Template und -Primer, Primer für Sequenzierung oder PCR-Screening und ein Expressionskontrollplasmid. WebGeneral Description DNA pTrcHis-TOPOfi Entire molecule length: 4390 bp Restriction Map Enzyme # of cuts Positions AccI 1 2966 AciI 62 237(c) 267 285(c) WebJan 3, 2007 · 1 Introduction. Vitamin B 6 is the collective term for a group of three related compounds, pyridoxine (PN), pyridoxal (PL) and pyridoxamine (PM), and their phosphorylated derivatives, pyridoxine 5′-phosphate (PNP), pyridoxal 5′-phosphate (PLP) and pyridoxamine 5′-phosphate (PMP). All organisms must either produce PLP or acquire it … modern physics by serway moses and moyer pdf