Phospholipidosis assay

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August undefined, 2024

WebMar 1, 2024 · The EM method, the gold standard of detecting cellular phospholipidosis, has downsides, namely, low-throughput, high-costs, and unsuitability for screening a large … WebMar 17, 2024 · The morphological hallmark of phospholipidosis is the appearance of unicentric or multicentric-lamellar bodies when viewed under an electron microscope (EM). The EM method, the gold standard of detecting cellular phospholipidosis, has downsides, namely, low-throughput, high-costs, and unsuitability for screening a large chemical library.

Adaptation of an In Vitro Phospholipidosis Assay to an Automated …

WebIn this assay, phospholipids (such as lecithin, lysolecithin and sphingomyelin) are enzymatically hydrolyzed to choline which is determined using choline oxidase and a H 2 … WebJun 21, 2024 · 1. Introduction. Drug-induced phospholipidosis (DIPLD) is characterized by excessive intracellular accumulation of phospholipids as a result of drug treatments [1].Since the first DIPLD case reported in 1948 [2], an increasing number of drugs were identified as PLD inducers, including antibiotics, antidepressants, antipsychotics and so … shark frenzy venice

Cell-Based Imaging Assay for Detection of Phospholipidosis.

WebDrug-induced phospholipidosis and steatosis can be determined using High Content Screening. Measuring multiple parameters from a single analysis, allows the simultaneous determination of changes in cell loss, nuclear size and intensity, and the presence of … WebPhospholipidosis is a lysosomal storage disorder characterized by the excess accumulation of phospholipids in tissues, often stimulated by drugs. Drug induced hepatotoxicity is a … WebThe EM method, the gold standard of detecting cellular phospholipidosis, has downsides, namely, low-throughput, high-costs, and unsuitability for screening a large chemical library. This chapter describes a cell-based high-content phospholipidosis assay using the LipidTOX reagent in a high-throughput screening (HTS) platform. popular delivery services

Phospholipidosis - an overview ScienceDirect Topics

Traduction de "monitored by assay" en français - Reverso Context

WebJun 22, 2024 · To investigate the role of phospholipidosis in antiviral activity in vitro, we tested 19 drugs for their induction of this effect in A549 cells using the well-established … WebeCiphr ® Cardio — iPS cell-derived cardiomyocytes and MEA. Structural cardiotoxicity assay — using 3D spontaneously beating microtissues. Combined hypertrophy and cardiotoxicity assay — using 3D spontaneously beating microtissues. Cardiotox screen — … popular dentists near meWebMar 5, 2015 · Synthesis and phospholipidosis effect of a series of cationic amphiphilic compounds: a case study to evaluate in silico and in vitro assays Article Full-text available shark french bulldog

"WebSuch assays rely heavily on the development prospective assays aim to predict toxicities that could lead to the ter- of human physiologically relevant model systems in combination with mination of a drug candidate or an entire development programme, appropriate toxicity end points. and bespoke retrospective assays are used for issue resolution ... " - Phospholipidosis assay

Phospholipidosis assay

WebDec 1, 2011 · In summary, we demonstrated an in vitro phospholipidosis screening assay in CHO-K1 and HepG2 cells based on a high content screening analysis method that can … WebJul 2, 2008 · In vitro screening assays were developed in HepG2 cells, rat primary hepatocytes, and rhesus monkey hepatocytes using the fluorescent-labeled phospholipid probe N - (7-nitrobenz-2-oxa-1,3-diazol-4-yl)-1,2-dihexadecanoyl- sn -glycero-3-phosphoethanolamine (NBD-PE) or Nile Red lipid stain.

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WebWhen studying phospholipidosis/steatosis in vitro, IONTOX uses HepG2 cells in combination with LipidTox Red, as our primary model to discriminate between phospholipidosis … WebSep 3, 2013 · In this study, a cell-based phospholipidosis assay has been developed using the LipidTOX Red reagent in HepG2 cells and miniaturized into a 1536-well plate format. To validate this assay for high ...

WebTo identify compounds that may induce phospholipidosis early in the discovery process, we have developed a predictive fluorescent cell-based assay amenable to automated high …

WebCerep Phospholipidosis assay (HepG2 cells) PubChem. 2 Comment. Help. New Window. Compounds with activity <= 10uM or explicitly reported as active by ChEMBL are flagged as active in this PubChem assay presentation. Target ChEMBL ID: CHEMBL1626541. ChEMBL Target Name: Phospholipidosis. WebIn this study, a cell-based phospholipidosis assay has been developed using the LipidTOX Red reagent in HepG2 cells and miniaturized into a 1536-well plate format. To validate this assay for high-throughput screening (HTS), the LOPAC library of 1280 compounds was screened using a quantitative HTS platform. A group of known phospholipidosis ...

WebFluorescent images were captured and analyzed using the Discovery-1 automated cellular imaging system. Eleven out of the 12 selected positive compounds and all negative compounds were properly assigned as positive and negative inducers of phospholipidosis, respectively, indicating the high degree of sensitivity and specificity of this assay.

WebEchelon’s LPLA2 Inhibitor Screen is a high throughput assay designed to screen drugs abilitiy to inhibit LPLA2 activity in vitro, a potential predictor of drug-induced phospholipidosis. Sample Volume: 1 μL. Sample Number: 128 Samples (in triplicates)/384-well plate. Echelon’s LPLA2 Inhibitor Screen (K-7000I) is designed to assay a drug’s ... popular designer boots 2021WebMembrane permeability assays demonstrate that the alkaloids are biologically available, although fangchinoline showed lower permeability than tetrandrine. At high concentrations, the extract, alkaloid fractions, and pure alkaloids induced phospholipidosis in 293TAT cells and less so in VeroAT cells. Gene expression profiling during virus ... shark frenzy movieWebJun 12, 2007 · Using an expanded set of compounds, we show that this assay correctly identified 100% of PLD-positive and -negative compounds. Dose-dependent increases in intracellular fluorescent phospholipid accumulation were observed. We found that this assay was less time consuming, more sensitive, and higher throughput than gene expression … shark friendship braceletWebThe LipidTOX assay series includes neutral lipid stains for fixed cells and phospholipid stains for live cells, which can be combined in multiplexed assays. Automatically … shark frozenWebTraductions en contexte de "monitored by assay" en anglais-français avec Reverso Context : If self-injection with Improvac is suspected, reproductive physiology should be monitored by assay of testosterone or oestrogen levels (as appropriate). The risk shark friend angel martha maggioWebDec 1, 2011 · Phospholipidosis is a lipid storage disorder which is recognized by excessive accumulation of phospholipids in the lysosomes within cells. Phospholipidosis can result from hereditary predisposition, which is for example the case in Niemann-Pick disease, but may also be the result of drug exposure. shark front license plateWebcontent phospholipidosis assay using the LipidTOX reagent in a high-throughput screening (HTS) plat- form. This assay has been optimized and validated in HepG2 and HepRG cells, and miniaturized into a popular designer clothes and stores