P-irf3 antibody
Webb26 feb. 2024 · IRF3 is activated after phosphorylation by the kinases IKKi and TBK1. 23 To determine whether SARS-CoV-2 nsp12 antagonizes IRF3 phosphorylation, 293T cells were transiently transfected with a... WebbDsRNA generated during the course of an viral infection leads to IRF3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to …
P-irf3 antibody
Did you know?
Webb23 mars 2024 · IRF-3 Antibody (FL-425) has been replaced by a more specific monoclonal antibody, IRF-3 (SL-12) ... Our IRF3 antibody sc-9082 will detect phosphorylated and unphosphorylated forms of IRF3. Answered by: Technical Service. Date … Webb8 mars 2024 · (C) Endogenous JMJD6 interacts with IRF3 or p-IRF3 after a viral infection or poly(I:C) stimulation. HEK293T cells were infected with SeV for 12 h. Cells lysates were immunoprecipitated with anti-IRF3 or anti-p-IRF3, and the immunoprecipitates were analyzed by immunoblot with anti-JMJD6 antibody (top panel).
WebbFC experiment of HepG2 using 11312-1-AP. 1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human IRF3 (11312-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit … Webb24 apr. 2014 · Anti-TRAF3 antibody was used to detect the existence of TRAF3 in the enriched Flag-IKKε. (E) HSCARG impairs IRF3 phosphorylation. HEK293T cells transfected with vector or Flag-RNF5 or Flag-HSCARG were infected with SeV for 6 h, and then IRF3 phosphorylation was analyzed with p-IRF3 antibody. (F) HSCARG suppresses IRF3 …
Webb11 apr. 2024 · Cells were lysed after 1.5 h and IRF3 was immunoprecipitated with anti-IRF3 antibody (1:100 dilution, 655702, 12A4A35, BioLegend). The level of TBK1 co-immunoprecipitated was assessed with ... WebbAnti-IRF3 Antibody Products. Anti-IRF3 antibodies are offered by a number of suppliers. This target gene encodes the protein 'interferon regulatory factor 3' in humans and may also be known as IIAE7. Structurally, the protein is reported to be 47.2 kilodaltons in mass. Based on gene name, canine, porcine, monkey, mouse and rat orthologs may ...
WebbAll lanes : Anti-IRF3 antibody [EPR2418Y] (ab68481) at 1/1000 dilution Lane 1 : Wild-type HAP1 cell lysate Lane 2 : IRF3 knockout HAP1 cell lysate Lane 3 : HeLa cell lysate Lane 4 : Jurkat cell lysate Lysates/proteins at 20 µg per lane. Predicted band size: 47 kDa. Lanes 1 - 4: Merged signal (red and green).
WebbThese data support that TBK1-induced chemokines promote both CD8 + T cell and Treg cell infiltration into TE def+ tumors and that TBK1-mediated interferon signaling (e.g., IRF3 and IRF7) causes CD8 + T cell exhaustion through inducing PD-L1 expression on tumor cells, which is in line with the reported role of IRF3/7 in induction of PD-L1 expression. … bps cbt trainingWebbIRF3 antibody Provider product page Proteintech Group - 11312-1-AP Antibody type Polyclonal Description KD/KO validated IRF3 antibody (Cat. #11312-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: FC, IF, IHC, IP, WB, ELISA. Reactivity Human, Mouse Host Rabbit gyn com tonicaWebbIRF3 [p Ser386] Antibody (EPR2346) Summary Immunogen A phospho-specific peptide corresponding to residues surrounding Serine 386 of human IRF3 was used as an … bpsc book pdfWebbAntibody was affinity purified using an epitope specific to IRF3 immobilized on solid support. The epitope recognized by A303-384A maps to a region between residue 375 and 419 of mouse Interferon Regulatory Factor 3 using the numbering given in entry NP_058545.1 (GeneID 54131). gyn columbus inWebbDuring infection, IRF-3 is likely activated through a pathway that includes activation of Toll-like receptors and of a kinase complex that includes IKKε and TBK1 (6,7). IRF-3 is … bpsc cce pyqWebb3 maj 2016 · The membranes were then analyzed for the expression of p65, p-p65, IRF3, and p-IRF3 proteins by immunoblotting using rabbit anti-p65, anti-p-p65, anti-IRF3, and anti-p-IRF3 antibodies, respectively. An anti-PDCoV N protein monoclonal antibody was used for immunoblotting to confirm the expression levels of the PDCoV N protein. gyn co testingWebb"IRF3 antibodies" comparison. At Proteintech, we pride ourselves on our antibody quality, customer service and transparency. As such, we are comparing our antibodies with … bpsc cdpo full form