Cyto-id® green detection reagent
WebThe Cyto-ID™ Autophagy Detection Kit is a member of the CELLestial® product line, reagents and assay kits comprising fluorescent molecular probes that have been … Web- 2 µL of CYTO-ID® Green Detection Reagent and 1 µL of Hoechst 33342 Nuclear Stain for every 1 mL of 1X Assay Buffer or complete cell growth medium: Downstream tips ; Incase of Low CYTO-ID Green dye staining in all treatments, increase the reagent concentration (500X dilution of the dye is recommended) and the incubation time. ...
Cyto-id® green detection reagent
Did you know?
WebNUCLEAR-ID ® Green chromatin condensation detection kit : A highly permeable green-emitting dye for enhanced detection of apoptosis … WebNovel Green Plus (20,000X) DNA Staining Reagent: 500 μl: AGT002-0500: Agarose Tablets, 110 pcs: 0.5g x 110: ... COVID-19 RT-qPCR Detection Kit Plus (TFDA) 100 rxns: ... 【CYTO-ID ®自噬檢測試劑盒 】 Autophagy detection kit貨號ENZ-51031-K200
WebDec 22, 2024 · The cell residues were washed twice with the assay buffer, and 100 μl of Dual Detection Reagent (2 μl of Cyto-ID Green Detection Reagent and 1 μl of Hoechst 33,342 Nuclear Stain in 1 mL of cell culture medium) was then dispensed in each well. The cells were protected from light and incubated at 37°C for 30 min. Web1. Product and Company Identification Product Code: 10-4410-xx Product Name: UniCap Phosphoramidite Product Use: For Research Use Only Manufacturer Information Company Name: Glen Research LLC 22825 Davis Drive Sterling, VA 20164 USA Phone Number: 1-703-437-6191 Web site address: www.glenresearch.com Email address: …
WebCYTO-ID® Autophagy Detection Kit 10662-266 / 89165-926 Dyes, Rapamycin, Chloroquine, 10x assay buffer CYTO-ID® Autophagy Detection Kit 2.0 75802-912 / 75802-914 Dyes, Rapamycin, Chloroquine, 10x assay buffer Dyes Included in Kit Excitation/Emission Platform Cell Type CYTO-ID® Green Detection Reagent Hoechst … WebIn brief, cells were washed with PCR PBS (supplemented with 5% FBS) and then Gene Primer sequence mixed with Cyto-ID Dual Detection Reagent solution (Cyto-ID Green Detection Reagent and GAPDH Hoechst 33342).
WebAug 6, 2024 · Autophagy analysis was performed by incubating cells with bafilomycin A1 for 30 min at 37 °C prior to treatment with CYTO-ID Green Detection Reagent, and then analyzing fluorescence by flow cytometry using the …
http://biopioneer.com.tw/?p=41098 shut ins milton flWebOct 1, 2024 · The autophagy level of haemocytes was detected with CYTO-ID® Autophagy Detection Kit (Enzo, Switzerland) according to the instruction. ... for 3 h, and then washed two times with M-L15 medium. The haemocytes on the slide were incubated with 200 μL CYTO-ID® Green Detection Reagent (diluted 1:500 in M-L15 medium) at 37 °C in dark … the paddock golf domeWebReagents Hypoxia Red Detection Reagent, part of the Cyto-ID® Hypoxia/Oxidative Stress Detection Kit (Catalog No. ENZ-51042) was provided by Enzo Life Sciences (Farmingdale, NY). The reagent is a weakly-fluorescent compound containing a nitro (NO 2) moiety. Due to nitroreductase activity present in hypoxic the paddock fort worthWebApr 9, 2024 · CYTO-ID ® Green Detection Reagent 2 was stained for 30 min and analyzed by flow cytometry. Compared with untreated cells, the fluorescence was increased, with treatment with RAP + CLQ (Figures 4J,K). The results indicated that autophagy can be induced under stress conditions. the paddock guisboroughWebSep 2, 2024 · When the cells reached 90% confluence, the medium was removed and replaced with 100 µL per well of Microscopy Dual Detection Reagent containing 1 µL of CYTO-ID ® Green Detection Reagent 2 and 1 µL Hoechst 33342 Nuclear Stain mixed in 1 mL neurobasal medium without Phenol Red Indicator, supplemented with 5% FBS. The … shut ins mo to murrayWebJul 16, 2014 · Cyto-ID Green Detection Reagent Kit (Enzo Life Sciences, Farmingdale, NY) detects autophagosomes and autolysosomes as recommended by manufacturer . In … shut ins nhtsWebFeb 14, 2024 · Cyto-ID green detection reagent, consisting of 1 μL Hoechst 33342, 100 μL Cyto-ID reagent per 1 mL cell culture medium supplemented with 10% FBS was used to stained cells. The plate was stored ... shut ins netflix